| Spigelia chiapensis K. Gould, 1999 |
| Spigelia coelostylioides K. Gould, 1999 |
| Spigelia coulteriana Benth., 1857 |
| Spigelia dolichostachya Fern. Casas, 2004 |
| Spigelia elbakyanii S. Islas & L.O. Alvarado, 2020 |
| Spigelia guerrerensis L.O. Alvarado & J. Jiménez Ram., 2015 |
| Spigelia hedyotidea A. DC., 1845 |
| Spigelia humboldtiana Cham. & Schltdl., 1826 |
| Spigelia longiflora Sessé & Moc., 1893 |
| Spigelia mexicana A. DC., 1845 |
| Spigelia nicotianiflora Chodat & Hassl., 1903 |
| Spigelia palmeri Rose, 1895 |
| Spigelia platyphylla Progel, 1868 |
| Spigelia polystachya Klotzsch ex Progel, 1868 |
| Spigelia pygmaea D.N. Gibson, 1968 |
| Spigelia scabra Cham. & Schltdl., 1826 |
| Spigelia scabrella Benth., 1840 |
| Spigelia speciosa Kunth, 1819 |
| Spigelia splendens H. Wendl. ex Hook., 1861 |
| Spigelia trispicata H. Hurley ex K. Gould, 1999 |