| Megachile peruviana Smith, 1879 |
| Megachile rhodopus Cockerell, 1896 |
| Megachile subexilis Cockerell, 1903 |
| Megachilidae Latreille, 1802 |
| Megachilinae Latreille, 1802 |
| Megachilini Latreille, 1802 |
| Osmia Panzer, 1806 |
| Osmia (Acanthosmioides) Ashmead, 1899 |
| Osmia (Acanthosmioides) nigrobarbata Cockerell, 1916 |
| Osmia (Cephalosmia) Sladen, 1916 |
| Osmia (Cephalosmia) marginipennis Cresson, 1878 |
| Osmia (Chalcosmia) Schmiedekneecht, 1885 |
| Osmia (Chalcosmia) coloradensis Cresson, 1878 |
| Osmia (Chalcosmia) georgica Cresson, 1878 |
| Osmia (Chalcosmia) texana Cresson, 1872 |
| Osmia (Chenosmia) Sinha, 1958 |
| Osmia (Chenosmia) gaudiosa Cockerell, 1907 |
| Osmia (Chenosmia) laeta Sandhouse, 1924 |
| Osmia (Diceratosmia) Robertson, 1903 |
| Osmia (Diceratosmia) aliciae Ayala & Griswold, 2005 |