| Neoprosena Townsend, 1927 |
| Neopsalida Townsend, 1916 |
| Neoptera Wulp, 1890 |
| Neosolieria Townsend, 1927 |
| Neosolieria sila (Reinhard, 1958) |
| Neotrafoia Townsend, 1912 |
| Nicetria Reinhard, 1953 |
| Nigrilypha O'Hara, 2002 |
| Nigrilypha gnoma O'Hara, 2002 |
| Nimioglossa Reinhard, 1945 |
| Nimioglossa ravida Reinhard, 1945 |
| Oberonomyia Reinhard, 1964 |
| Oberonomyia palpalis Reinhard, 1964 |
| Ocypteryx Townsend, 1931 |
| Ocyrtosoma Townsend, 1912 |
| Ocyrtosoma rufum (Brauer & Bergenstamm, 1893) |
| Oedematocera Townsend, 1916 |
| Oestrogaster Townsend, 1912 |
| Oestrogastrodes Townsend, 1915 |
| Oestrogastropsis Townsend, 1915 |