| Allophallus Hubbs, 1936 |
| Allophallus kidderi Hubbs, 1936 |
| Belonesox Kner, 1860 |
| Belonesox belizanus Kner, 1860 |
| Belonesox belizanus subsp. maxillosus Hubbs, 1936 |
| Brachyrhaphis Regan, 1913 |
| Brachyrhaphis hartwegi Rosen & Bailey, 1963 |
| Carlhubbsia Whitley, 1951 |
| Carlhubbsia kidderi (Hubbs, 1936) |
| Dicerophallus Álvarez, 1952 |
| Dicerophallus echeagarayi Álvarez, 1952 |
| Gambusia Poey, 1854 |
| Gambusia affinis (Baird & Girard, 1853) |
| Gambusia alvarezi Hubbs & Springer, 1957 |
| Gambusia atrora Rosen & Bailey, 1963 |
| Gambusia atzi Rosen, 1951 |
| Gambusia aurata R. R. Miller & W. L. Minckley, 1970 |
| Gambusia bonita Meek, 1904 |
| Gambusia echeagarayi (Álvarez, 1952) |
| Gambusia eurystoma R. R. Miller, 1975 |