| Gambusia fasciata Meek, 1904 |
| Gambusia gracilis Girard, 1859 |
| Gambusia heckeli Bleeker, 1860 |
| Gambusia humilis Günther, 1866 |
| Gambusia hurtadoi Hubbs & Springer, 1957 |
| Gambusia infans Woolman, 1894 |
| Gambusia krumholzi Minckley, 1963 |
| Gambusia longispinis Minckley, 1962 |
| Gambusia luma Rosen & Bailey, 1963 |
| Gambusia marshi Minckley & Craddock, 1962 |
| Gambusia milleri (Radda, 1987) |
| Gambusia modesta Troschel, 1865 |
| Gambusia panuco Hubbs, 1926 |
| Gambusia plumbea Troschel, 1865 |
| Gambusia quadruncus Langerhans, 2012 |
| Gambusia rachovii (Regan, 1914) |
| Gambusia rachowi (Regan, 1914) |
| Gambusia regani Hubbs, 1926 |
| Gambusia rhizophorae Rivas, 1969 |
| Gambusia senilis Girard, 1859 |