| Gambusia sexradiata Hubbs, 1936 |
| Gambusia speciosa Girard, 1859 |
| Gambusia turrubarensis Meek, 1912 |
| Gambusia vittata Hubbs, 1926 |
| Gambusia yucatana Regan, 1914 |
| Gambusia yucatana subsp. australis Greenfield, 1985 |
| Gambusia zarskei M. K. Meyer, Schories & Schartl, 2010 |
| Girardinus Poey, 1854 |
| Girardinus pleurospilus Günther, 1866 |
| Girardinus sonoriensis Girard, 1859 |
| Glaridodon Garman, 1895 |
| Glaridodon latidens Garman, 1895 |
| Heterandria Agassiz, 1853 |
| Heterandria affinis Baird & Girard, 1853 |
| Heterandria bimaculata (Heckel, 1848) |
| Heterandria jonesii (Günther, 1874) |
| Heterandria lutzi Meek, 1902 |
| Heterandria occidentalis Baird & Girard, 1853 |
| Heterandria patruelis Baird & Girard, 1853 |
| Heterandria tuxtlaensis McEachran & DeWitt, 2008 |